ABSTRACT
Trata-se de um estudo de revisão integrativa que tem por objetivo analisar o levantamento das produções publicadaspela enfermagem brasileira acerca do suicídio na adolescência. Foram analisadas as bases de dados LILACS, MEDLINE,SciELO, BDENF e Rev Enf. O levantamento abrangeu as publicações da enfermagem brasileira entre os anos 2000 e2014, sendo identificadas nove referências que compuseram a amostra do estudo. A avaliação dos trabalhospossibilitou a elaboração de três categorias de análise: 1. Quem é o adolescente que tenta o suicídio e quais os meiosutilizados; 2. Os motivos que levam o adolescente ao suicídio; 3. A atuação do enfermeiro na recuperação doadolescente que tenta suicídio. Os resultados destacam a multicausalidade do suicídio na adolescência, os fatores de risco para o suicídio e o papel do enfermeiro na assistência a este sujeito...
The objective of this integrative review study is to survey the works published by Brazilian nursing about suicide inadolescence. The LILACS, MEDLINE, SciELO, BDENF, and Rev Enf databases were analyzed. The survey coveredBrazilian nursing publications from 2000 to 2014; 9 references, which comprised the study sample, were identified.The assessment of the works enabled the development of three categories of analysis: 1 - Who is the teenager whoattempts suicide and what means did he/she use? 2 - The reasons teenagers commit suicide; 3 - The role of the nursein the recovery of the teenager who attempts suicide. The results highlight the multiple causes of suicide inadolescence, risk factors for suicide and the role of nurses in the care for the subject...
Un estudio de revisión integradora fue hecho con el objetivo de estudiar las producciones publicadas por la enfermeríabrasileña a respecto de casos de suicidio en la adolescencia. Los datos fueron obtenidos de las bases de datos LILACS,MEDLINE, SciELO BDENF y Rev Enf. La encuesta abarcó las publicaciones de enfermería brasileñas entre los años 2000a 2014. Se identificaron nueve referencias que compusieron la muestra del estudio. La evaluación del trabajo permitióel desarrollo de tres categorías de análisis: 1 - Quién es el adolescente que intenta suicidarse y cuáles son los mediosutilizados; 2 - Las razones que llevan el adolescente al suicidio; 3 - El papel del enfermero en la recuperación deladolescente que intenta suicidarse. Los resultados ponen de relieve las múltiples causas de suicidio en laadolescencia, factores de riesgo para el suicidio y el papel de los enfermeros en el cuidado de este sujeto...
Subject(s)
Humans , Male , Female , Adolescent , Adolescent , Nurses, Male , SuicideABSTRACT
Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.
Subject(s)
Animals , Mice , Actins/metabolism , Trypanosomatina/metabolism , Blotting, Western , Electrophoresis, Polyacrylamide Gel , Fluorescent Antibody Technique , Microscopy, Confocal , Protozoan Proteins/metabolismABSTRACT
The aim of the present study was to evaluate sterilizationof intraoral X-ray film holder bite blocks by immersionin 2% glutaraldehyde solution for 10 hours. Seventeen intraoral X-ray film holder bite blocks were inoculated in Tryptic Soy Broth media containing one of the tested microorganisms(S. aureus and B. subtilis) and were incubated at 37 °C for 48 hours. One bite block was used as a positive control. The other blocks were immersed in 2% glutaraldehydefor 10 hours and one block was used as a negative control. Fifteen bite blocks were removed and five of them were washed with 70% alcohol, five blocks were washed with distilled water and the others were washed in running water. Bacterial growth was observed after incubation. No bacterial growth was found on any block, however, on the blocks that were washed with running water and distilled water, various colonies of fungi were observed, while no growth was found on the blocks which underwent a final wash with 70% alcohol. The 2% glutaraldehyde solution was effective for the sterilization of intraoral X-ray film holder bite blocks when they were immersed for 10 hours using 70% alcohol during the last wash.